General information
Mutation Designer is a fast and
easy-to use web tool which can predict primers for whole
plasmid Site-Directed Mutagenesis. It is suitable for
QuickChange® Site-directed mutagenesis(SDM), Q5® Site-directed
mutagenesis and even several homemade Site-directed mutagenesis
kits and workflows. We offer two variants of the tool, pick the
one that suits your workflow from the links below:
1) Q5-Like Site-directed Mutagenesis:
FW primer has the mutation and the primers do not overlap. Primers require 5'-phosphorylation before ligation.
1) Q5-Like Site-directed Mutagenesis:
FW primer has the mutation and the primers do not overlap. Primers require 5'-phosphorylation before ligation.
2) QuickChange-like
Site-directed Mutagenesis:
Both the FW and REV primers have the mutation and user can choose if they want completely overlapping or partially overlapping primers. There are parameters to adjust the overlap (when you choose partially overlapping primers)
Both the FW and REV primers have the mutation and user can choose if they want completely overlapping or partially overlapping primers. There are parameters to adjust the overlap (when you choose partially overlapping primers)